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Quenching Constants of the Original EGFP and Its Mutant Proteins
| Tryptophan accessibility |
Chromophore accessibility |
|||
|---|---|---|---|---|
| KSV (M−1) | kq (108M−1 s−1) | KSV (M−1) | kq (108M−1 s−1) | |
| EGFP | 2.59 ± 0.03 | 6.5 ± 0.4 | 0.06 ± 0.01 | 0.23 ± 0.07 |
| EGFP/Arg96Cys | 3.24 ± 0.05 | 7.7 ± 0.5 | 0.05 ± 0.01 | 0.19 ± 0.06 |
| EGFP/Arg96Ser | 1.59 ± 0.01 | 5.9 ± 0.6 | 0.05 ± 0.02 | 0.30 ± 0.07 |
| EGFP/Arg96Ala | 1.25 ± 0.04 | 5.6 ± 0.8 | 0.06 ± 0.01 | 0.22 ± 0.07 |
