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Thermodynamic Parameters of GdmCl-Induced Unfolding of EGFP and Its Mutant Proteins, as Obtained from the Analysis of the Equilibrium Transitions
| m (kcal mol−1M−1) | D50% (M) | (kcal mol−1) | |
|---|---|---|---|
| EGFP | 2.1 ± 0.2 | 3.3 ± 0.1 | −7.0 ± 0.5 |
| EGFP/Arg96Cys | 2.0 ± 0.2 | 3.6 ± 0.1 | −7.3 ± 0.8 |
| EGFP/Arg96Ser | 1.0 ± 0.2 | 3.1 ± 0.1 | −2.8 ± 0.6 |
| EGFP/Arg96Ala | 1.1 ± 0.2 | 2.8 ± 0.1 | −3.4 ± 0.7 |
