Figure 1.

Schema of the generation of DCs in CLL patients. (Inset) Importance of monocyte purity in undisturbed generation of αDC1. (Upper inset) Typical yields of the DCs generated from monocytes enriched by density gradient isolation (typically over 80% of contaminating CLL cells) and by magnetic isolation of CD14⁺ monocytes (monocyte purity, >90%). (Lower inset) Increasing concentrations of CLL cell were added to magnetically isolated CD14⁺ cells at the onset of DC cultures. In both cases, the yield of αDC1 is expressed as a fraction of the initial monocyte numbers.