Comparison of the phenotype of immature DCs (iDCs), as compared with αDC1 and sDCs matured in the absence or presence of γ-irradiated CLL cells. The expression levels of CD83, CD86, CD80, CD11c, and CD40 on αDC1 were higher than on sDCs (P<0.05). In contrast, CCR7 showed lower expression in αDC1 than sDCs. There was no significant impact of tumor loading upon the expression of these molecules in DCs. Data shown as mean fluorescence intensity (MFI) increase over isotype control ± sd (n=5 donors).