Figure 5.

Autologous tumor-loaded αDC1 are superior inducers of tumor-specific CD8⁺ T cells during in vitro sensitization (IVS). CTLs generated in three time-restimulated IVS cultures of CD8⁺ T cells were used as responder cells against autologous CLL cells. The numbers of IFN-γ-secreting, tumor-reactive CD8⁺ T cells were measured using IFN-γ ELISPOT. Anti-MHC I-blocking antibody was used to confirm the MHC class I dependence of CLL cell recognition. (A) Data from a single representative experiment showing the absolute numbers of tumor-reactive CD8⁺ T cells induced by tumor-loaded αDC1 or by tumor-loaded sDCs. (B) Data from four separate experiments (individual circles) using the cells of four different CLL donors are expressed as the ratios between the numbers of tumor-reactive CD8⁺ T cells induced by αDC1 and sDCs.