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Syncoilin disrupts the insolubility of Per45 in peripherin-transfected cells. Immunoblots of SW13vim(−) cells transfected with (A) Peripherin, Per45 or Per58 and (B) Sync2 with peripherin, Per45 or Per58. Cell lysates were fractionated, and the insoluble (I) and soluble (S) fractions were run separately. Ponceau loading controls are shown to verify equal loading. The blot in B was stripped and re-probed for syncoilin to verify the cells were co-transfected.
