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. 2010 Jul 6;123(15):2596–2604. doi: 10.1242/jcs.066514

Fig. 4.

Fig. 4.

p38γ regulates hDlg-PSF association independently of its catalytic activity. (A) WT, p38γ171A/171A or p38γ/δ−/− MEFs were treated as in Fig. 3C and endogenous hDlg immunoprecipitated using anti-hDlg antibody from 0.2 mg of protein lysate. Pellets were immunoblotted with anti-PSF or anti-hDlg antibody. Similar results were obtained in two independent experiments. (B) HEK293 cells were transfected with empty GFP-vector, GFP-p38γ(FL), GFP-p38γ(ΔC) or GFP-p38γ(KD). Endogenous hDlg was immunoprecipitated as in A, and pellets were immunoblotted with anti-PSF or anti-hDlg. (C) Cells were transfected as in B and exposed to 0.5 M sorbitol (15 minutes). Endogenous hDlg was immunoprecipitated, and pellets were immunoblotted with anti-P-hDlgS158 or anti-hDlg. Similar results were obtained in at least two independent experiments. Levels of p38γ expression in B and C were checked by immunoblotting 20 μg cell extract with anti-p38γ antibody.