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. 2010 Jul 6;123(15):2632–2639. doi: 10.1242/jcs.061606

Fig. 1.

Fig. 1.

Pax3 expression in C2C12 cells inhibits myogenic differentiation. (A) Western blot of C2C12 cells infected with the indicated retroviruses. (B) Bright-field photomicrographs of C2C12 cells infected with the indicated retroviruses, grown for 5 days, and then switched to differentiation medium for the indicated times. MyHC staining of C2C12 cells 2 days after switch to differentiation medium is shown on the right. Scale bars: 150 μm. (C) Quantification of results shown in B (4 days; n=3 replicates/experiments). (D) Proliferation curve of C2C12 cells infected with the indicated retroviruses, assessed by cell counting. (E) Percentage cell death of C2C12 cells infected with the indicated retroviruses, assessed by Trypan Blue staining. *P<0.01. Results are means ± s.e.m.