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. 2010 Jul 6;123(15):2632–2639. doi: 10.1242/jcs.061606

Fig. 3.

Fig. 3.

Endogenous expression of Pax3 in SMPs. (A) RT-PCR (non-quantitative) for Pax3 in freshly isolated hindlimb SMPs and SMPs transduced with Pax3 for 3 days. (B) Relative endogenous Pax3 mRNA levels in freshly sorted hindlimb SMPs (day 0), followed by in vitro culture for the indicated times. (C) Comparison of Pax3 mRNA levels by real-time RT-PCR (quantitative) in freshly isolated hindlimb, triceps and diaphragm SMPs. **P=0.07; *P<0.01. (D) Relative endogenous Pax3 mRNA levels in freshly sorted triceps SMPs (day 0), followed by in vitro culture for the indicated times. (E) Spontaneous differentiation of hindlimb vs triceps SMPs, as assessed by the percentage of elongated cells, 3 days (left) and 6 days (right) after isolation (n=3). *P<0.01; **P<0.05.