Table 1.
Methods and results of immunohistochemistry
| Antibody | Source | Dilution/antigen retrieval | Detection method | Reactivity |
|---|---|---|---|---|
| CK AE1/AE3 | Dako | 1:200/protease digestion | SA/Bi | - |
| CK7 | Dako | 1:200/protease digestion | SA/Bi | - |
| CK20 | Dako | 1:500/protease digestion | SA/Bi | - |
| TTF1 | Dako | 1:100/CC1/95°C/30 min | SA/Bi | - |
| EMA | Dako | 1:200/CC1/95°C/30 min | SA/Bi | - |
| Vimentin | Biogenex | 1:10000/CC1/95°C/30 min | SA/Bi | - |
| CD20 | Dako | 1:250/CC1/95°C/30 min | SA/Bi | - |
| CD30 | Dako | 1:50/CC1/95°C/30 min | SA/Bi | - |
| CD34 | Cell Marque | 1:50/CC1/95°C/30 min | SA/Bi | - |
| Kappa light chain | Dako | 1:5000/protease digestion | SA/Bi | - |
| Lambda light chain | Dako | 1:10000/protease digestion | SA/Bi | - |
| CD68 | Dako | 1:800/CC1/95°C/8 min | SA/Bi | - |
| S-100 | Univ. of Toronto | 1:1000/CC1/95°C/30 min | SA/Bi | - * |
| HMB-45 | Dako | 1:100/untreated | SA/Bi | - |
| Melan-A | Cell Marque | 1:50/CC1/95°C/30 min | SA/Bi | - * |
| PNL2 | Private source | RTU/untreated | Polymer based | - |
| SMA | Dako | 1:200/untreated | SA/Bi | - |
| Desmin | Dako | 1:200/CC1/95°C/30 min | SA/Bi | +++ |
| H-Caldesmon | Dako | 1:200/CC1/95°C/30 min | SA/Bi | - |
| Myogenin | Dako | 1:50/CC1/95°C/60 min | Polymer based | - |
| Myo-D1 | Dako | 1:60/citrate buffer (pH6.0) | Polymer based | - |
| CD99 | Signet | 1:20/CC1/95°C/30 min | SA/Bi | +++ |
| Synaptophysin | Cell Marque | 1:250/CC1/95°C/30 min | SA/Bi | +++ |
| Fli1 | BD Pharmingen | 1:20/CC1/95°C/60 min | Polymer based | - |
| WT1 | Dako | 1:50/CC1/95°C/60 min | Polymer based | - |
| Ki 67 | Lab Vision | 1:200/CC1/95°C/30 min | SA/Bi | >10% |
Dako, Carpinteria, CA, USA; Biogenex, San Ramon, CA, USA; Cell Marque, Rocklin, CA, USA; Signet, Dedham, MA, USA; BD Pharmingen, Franklin Lakes, NJ, USA.
SA/Bi: Streptavidin/biotin method; RTU: ready to use; CC1: Cell conditioning 1 (pH8.0) (Ventana medical systems, Tucson, AZ, USA).
*Negative on the core and open biopsies, but patchy positive staining was identified in the resection specimen.