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. 2010 Jun 18;9:155. doi: 10.1186/1476-4598-9-155

Figure 4.

Figure 4

The involvement of LMP1 induced Id1 in suppressing TGFβ downstream signalling. (A) HEK-293 cells were transfected with the SMAD-responsive luciferase reporter constructs: pGL3(CAGA) or p3TPlux together with various doses of an Id1 expression vector (pCDNA3-Id1). (B) HEK-293 cells were transfected with pGL3(CAGA) or p3TPlux and various doses of LMP1 expression vector together with Id1 shRNA (shId1B+C) or control scrambled shRNA. (C) HEK-293 cells were transfected with pGL3(CAGA) or p3TPlux and various doses of LMP1 expression vector together with or without a Foxo3a expression vector (pGFP-Foxo3a). Twenty-four hours post-transfection, cells were treated with 10 ng TGFβ in medium with 0.2% FBS for 16 hrs prior to harvesting and luciferase analysis.