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. 2010 Jul 21;5(7):e11672. doi: 10.1371/journal.pone.0011672

Figure 5. Inhibition of Akt activation in cells infected with parental virus leads to a phenotype closely resembling the phenotype of the ORF4 mutant virus.

Figure 5

NIH3T3 cells were treated with the indicated doses of Triciribine (TCN), or as a control, with DMSO, starting 1 h prior to infection. Then, cells were infected at a multiplicity of infection of 5–10. After 1h at 37°C, the inoculum was removed and fresh medium, with TCN or DMSO, respectively, was added. Cells were harvested after 18 hours and analyzed for gfp expression by FACS analysis (panel A), or were analyzed after 1.5 hours by microscopy using a 100× oil immersion objective (panel B) as described in Figure 3. Data shown in panel A are means ± SD of four independent experiments. The asterisk indicates a statistically significant difference of p = 0,005, and the two asterisks indicate a statistically significant difference of p = 0,0003 (Student's t-test). In panel B, one representative experiment of 2 is shown.