Table 1.
Random libraries containg various combinations of mutations
| Template | No. of Primers | Colonies Screened | Mutation Frequency | No. of Clones Sequenced | No. of mutations |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 or more | |||||
| MCon3 | 7 | 92 | 73.9%a | 33 | 0 | 0 | 1 | 5 | 7 | 12 | 6 | 2 | ||||||
| MCon3 | 10 | 96 | 85.4%b | 96 | 14 | 5 | 9 | 18 | 18 | 14 | 13 | 3 | 1 | 1 | 0 | |||
| MCon3 | 24 | 192 | 79.2%a | 32 | 0 | 0 | 0 | 9 | 10 | 1 | 3 | 3 | 2 | 1 | 2 | 0 | 1 | 0 |
| WEAU.WT6 | 36 | 96 | 93.8%b | 96 | 6 | 14 | 21 | 17 | 16 | 9 | 9 | 2 | 1 | 1 | 0 | 0 | 0 | 0 |
a
Percentage of clones showing Env protein band at increased size by Western blot analysis
b
Percentage of clones with mutations by directly DNA sequencing screening.