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Multiple mutations introduced by overlapping primers in individual clones
| Template | No. of primers in the mutagenesis reaction | Mutant ID | Mutations generated by overlapping primers |
|---|---|---|---|
| MCon3 | 24 | 3F4 | S224N+S231N |
| 3F9 | S279N+S285N+S291N | ||
| 3B2 | S343N+S349N | ||
| 3D7 | S383N+S386N | ||
| WEAU.WT6 | 36 | 1H2 | M46I+I54M |
| 1G5 | I47V+I54M | ||
| 1C8 | L76V+V82A | ||
| 1B1 | L76V+V84A | ||
| 1F2 | L76V+V82A, L210W+K219Q | ||
| 1A7 | L210W+K219Q | ||
| 1B11 | L210W+K219Q | ||
| 1C4 | L210W+K219Q | ||
| 1C5 | L210W+K219Q | ||
| 1C7 | L210W+K219Q | ||
| 1F1 | T215Y+P225H | ||
