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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Immunobiology. 2010 Mar 16;216(1-2):66–71. doi: 10.1016/j.imbio.2010.03.003

Figure 4. Analysis of the early expression of the CD69 activation marker in Tgr+ cells from young and old AND mice shows that age and OSGE affect the early expression of the CD69 activation marker in cells that have not divided.

Figure 4

A- 16 hours after adoptive transfer, untreated and OSGE-treated Trg+ cells were gated and analyzed as described in Figure 1, but with the addition of CD69 staining and analysis. No cell division was found using the CFSE+ as a marker of proliferation. The crossbar in each dot-plot represents the threshold used to discriminate CD69+ from CD69 negative cells in each peak defined by CFSE expression. B-The untreated and OSGE treated Trg+ cells corresponding to undivided cells were quantified from matched sets of 2 young (open bars) and 1 old (filled bars) in each of 4 experiments. Each bar shows mean percentage ± SEM. The * indicates statistical significance with respect to young cells not exposed to OSGE, and the ^ represents significant differences with respect to old cells not exposed to OSGE.