Figure. 1.

Western blot analysis showing differential levels of immunoreactivity with different parkin antibodies in various mouse strains. Cerebral cortical tissues were harvested from Balb/c, C57BL/6 (BL6), C3H, and 129S mice and total protein lysates were extracted. Equal amounts (18 ug) of total protein extracts were loaded on 13% polyacrylamide gels and analyzed by western blot analysis with the anti-parkin monoclonal antibodies, PRK8, PRK28, and PRK109 as well as an actin antibody to confirm equal protein loading. The representative blot above shows extracts from 10 week old mice. Two samples were loaded, each from separate mice of the indicated strains. All experiments were replicated and similar results were obtained for a total of n=4 mice from each strain.