Table I.

Oligonucleotide sequences used to generate N-and C- terminal truncated human parkin mutants for PRK8, PRK28 and PRK109 epitope mapping studies.

Nucleotides amplified in PCR	Corresponding Amino Acid Sequence	Forward Oligonucleotide sequence	Reverse Oligonucleotide sequence
1–1398	1–465	5’-ATG ATA GTG TTT GTC AGG TTC AAC TCC-3’	5’- CTA CAC GTC GAA CCA GTG GTC CCC CAT -3’
1–1260	1–420	5’-ATG ATA GTG TTT GTC AGG TTC AAC TCC-3’	5’-CTA GCG GGG ACA GGG CTT GGT GGT TTT CTT-3’
1–1224	1–408	5’-ATG ATA GTG TTT GTC AGG TTC AAC TCC-3’	5’-CTA TTT GGA GGC TGC TTC CCA ACG AGC CTG-3’
1–1197	1–399	5’-ATG ATA GTG TTT GTC AGG TTC AAC TCC-3’	5’-CTA CTC GGC GGC TCT TTC ATC GAC TCT GT-3’
661–1398	221–465	5’-GAA ACA CCA GTA GCT TTG CAC CTG AT- 3’	5’- CTA CAC GTC GAA CCA GTG GTC CCC CAT -3’
1141–1398	381–465	5’-TTT GAA GCC TCA GGA ACA ACT ACT CAG-3’	5’- CTA CAC GTC GAA CCA GTG GTC CCC CAT -3’
1195–1398	399–465	5’-GAG CAG GCT CGT TGG GAA GCA GCC-3’	5’- CTA CAC GTC GAA CCA GTG GTC CCC CAT -3’