Skip to main content
. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Mol Microbiol. 2010 May 12;76(6):1539–1554. doi: 10.1111/j.1365-2958.2010.07184.x

Figure 2. A-motility analysis of M xanthus strains DZ2 (wt), frzCD, aglZ and agmU mutants.

Figure 2

In the S− background (pilA), single cells swarming out from the edge of colonies on 1.5% agar (or agarose) were monitored as an indicator of A-motility (Hodgkin, 1979). Cells (10 µl), at a concentration of 4 × 109 cfu ml−1, were spotted on CYE plates containing 1.5% (w/v) agar (or agarose), incubated at 32°C and photographed after 48 h with a WTI charge-coupled device (CCD)-72 camera, using a Nikon Labphot-2 microscope. Scale bar, 40 µm.