Fig. 3. B cells are not required for arthritis development in TS1×HACII mice.
(A) Histograms show CD86 and MHC class II expression on B220+ splenocytes from TS1 (grey lines), TS1×HA104 (dashed lines), and TS1×HACII (black lines) mice. (B) Bar graphs show the numbers of B220+ splenocytes and serum IgG with circles representing individual mice. Bars indicate the averages for TS1 (white bars), TS1×HA104 (light grey bars), and TS1xHACII (dark grey bars) mice. (C) Graph indicates the largest ankle widths from individual TS1.JH−/− (open bars) and TS1×HACII.JH−/− (filled bars) mice. The mean ankle width of TS1×HACII.JH−/− mice is significantly greater than that of TS1.JH−/− mice (P<0.01, Student's t-test). Mice used in this analysis ranged in ages from 6 to 23 weeks. Picture shows H&E stained knee section from an arthritic TS1×HACII.JH−/− mouse. p, pannus; arrowhead, bone erosion.
