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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Immunol Rev. 2010 Jan;233(1):97–111. doi: 10.1111/j.0105-2896.2009.00848.x

Fig. 6. Autoreactive CD4+ T cells in TS1×HACII mice are activated and produce cytokines.

Fig. 6

(A) Expression of 6.5 versus CFSE on purified CD4+ T cells from TS1, TS1×HA104, and TS1×HACII mice following 72 h of culture with or without S1 peptide and/or IL-2. (B) Amounts of indicated cytokines in culture supernatants obtained following incubation of unfractionated LN cells from individual TS1 (open bars), TS1×HA104 (grey bars), and TS1×HACII (filled bars) mice for 3 days in the absence of exogenous peptide or cytokines. Bars indicate means. (C) Dot plots show intracellular cytokine staining of CD4+ (upper panels) and 6.5+CD4+ (lower panels) lymph node cells from TS1×HACII mice following stimulation immediately ex vivo either with PMA and ionomycin for 4 h (for IL-17, IFN-γ, IL-10 and IL-4) or with 3 μM S1 peptide for 4 (for IL-6 and TNF-α). Percentages of cells in indicated gates are shown.