Fig. 7. TS1×HACII mice contain CD4+CD25+Foxp3+ Treg cells, including a population that recognizes the target autoantigen.

(A) Lymph node cells from TS1×HACII and TS1 mice were stained with CD4, CD25, and Foxp3. (B) Lymph node cells from a TS1×HACII mouse were stained with 6.5 to identify HA-specific cells, CD4, CD25, and Foxp3. (C) Lymph node cells from five, eight, and 12-week-old TS1×HACII mice were stained with CD4, CD25, and Foxp3. (D) Lymph node cells from TS1×HACII and BALB/c mice were stained with CD4, CD25, Foxp3, GITR, and CD103. A 6.5 stain was also included for TS1×HACII mice.