Figure 1.

c-myc promoter binding protein (MBP-1) upregulates miR-29b in prostate cancer cells. PC3 and DU145 cells were transduced with control dl312 or AdMBP-1 for 48 hours. Total RNA was isolated, and expression of miR-29b was measured using quantitative reverse transcription polymerase chain reaction with a hydrolysis probe and standardized to small nuclear RNA U6, which was used as the endogenous control. The results are presented from 3 independent experiments, mean ± standard error. P < 0.001 by 1-way analysis of variance.