The placental tissue (0.3 g) was homogenized in 1 ml RIPA lysis buffer. The homogenate was kept on ice for 30 min and the protein concentration of supernatant was determined. Cytokines concentrations were measured in the total protein fraction using ELISA. (A) TNFα measurements in normal, E.coli infected and G4-PAMAM-OH treated guinea pigs (B) IL-6 measurements in normal, E.coli infected and G4-PAMAM-OH treated guinea pigs (C) IL-1β measurements in normal, E.coli infected and G4-PAMAM-OH treated guinea pigs * P<0.05, *** P<0.001 Vs. Normal control. ▲▲ P<0.01, ▲▲▲ P<0.001 Vs. E.coli group.