Table 1.

DNAzyme sequences, data fitting, and melting temperatures. First-order rate constants (k) were fit to to [S]=A*(1-e^−kt), where [S] is the fraction of substrate uncleaved at timet, and A is the percentage of total RNA cleaved as time approaches infinity. Binding arms are in lowercase and catalytic loop in uppercase. PS indicates photocleavage site.

	Sequence	k (−UV) (min−1)	k (+UV) (min−1)	Photo- modulation[a]	A (−UV) (%)	A (+UV) (%)	Melting Temp. (°C)
Dz0	tgctctccaGGCTAGCTACAACGAcctgcacct	0.24(±0.03)	0.21(±0.02)	1:1	77(±2)	80(±2)	49
Dz1	tgctctccaGGCTAGC-PS-ACAACGAcctgcacct	0.089(±0.003)	0.039(±0.003)	2:1	70(±1)	76(±3)	49
Dz2	tgctctccaGGCTAG-PS-TACAACGAcctgcacct	Inactive	Inactive	N/A	-	-	49
Dz3	tgctctccaGGCTAG-PS-ACAACGAcctgcacct	Inactive	Inactive	N/A	-	-	49
Dz4	tgctctccaGGCTAGCTACAACGAcct-PS-cacct	0.055(±0.007)	0.032(±0.01)	2:1	53(±3)	21(±4)	39
Dz5	tgctc-PS-ccaGGCTAGCTACAACGAcctgcacct	0.12(±0.02)	0.077(±0.01)	2:1	81(±3)	31(±1)	39
Dz6	tgctc-PS-ccaGGCTAGC-PS-ACAACGAcctgcacct	0.069(±0.002)	Inactive	on→off	74(±1)	-	36
Dz7		0.022(±0.007)	0.21(±0.02)	1:10	58(±10)	79(±1)	43
Dz8		Inactive	0.024(±0.009)	off→on	-	56(±10)	N/D[c]
Dz1a	tgctctccaGGCTAGC	0.10(±0.02)[b]	N/A	N/A	51(±2)	N/A	39
Dz1b	5′ – PO42− - ACAACGAcctgcacct						39

^aPhotomodulation is the ratio of rate constants (k) before and after UV photolysis.

^bDz1a and Dz1b were inactive when tested alone. k value is for a 1:1 mixture of the two strands.

^cThe internal melting temperature of Dz8 alone was 74 °C, so its melting temperature with the target RNA was not determined.