Figure 2.

HIP14 siRNA affects in vitro palmitoylation of the FarnCNRas(NBD) peptide and subcellular localization of H-RAS-GFP. In vitro palmitoylation assays performed on membrane fractions from the indicated cell lines. Each bar represents three independent experiments and the error bars are the standard deviation. RT–PCR was performed using 1 μg of total RNA isolated from the indicated cell lines and primers that recognize both human and murine HIP3