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. Author manuscript; available in PMC: 2010 Jul 22.
Published in final edited form as: Exp Cell Res. 2009 Jul 4;315(18):3176–3186. doi: 10.1016/j.yexcr.2009.06.030

Fig. 5.

Fig. 5

Effect of selective removal of F-actin from detergent-insoluble pellet on Hsp27. (A) Western blot of Hsp27 and α-sarcomeric actin in the detergent-insoluble fraction of heat-shocked myocardium following incubations with PBS or 1 M NaSCN. (B) Quantitative analysis of Hsp27 (left) and sarcomeric actin (right) immunoblots following incubations with PBS or 1 M NaSCN in 4 independent experiments. (*) denotes statistically significant difference when compared to PBS treated samples. Error bars are the SEM. (C) Localization of Hsp27 (left) and F-actin (right) in detergent-insoluble fractions of heat-shocked adult zebrafish myocardium following treatment with PBS or 1 M NaSCN. Hsp27 (lower left) remains in a striated pattern associated with myofibrils after extraction of F-actin. Scale bars are 10 μm.