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. 2010 Jun 10;19(16):3254–3265. doi: 10.1093/hmg/ddq234

Figure 2.

Figure 2.

Motor neurons expressing mutant HSPB8 show enhanced neuritic APP accumulation. Rat motor neurons were transduced with pLenti-GFP, pLenti-WT-HSPB8-GFP or mutant pLenti-K141N/K141E-HSPB8-GFP constructs at DIV3 and immunostained using amyloid precursor protein (APP) antibody at DIV7. Motor neuron neurites expressing GFP (A) or HSPB8-WT (B) mainly show a faint and punctate appearance of APP in the neurites, similar to non-transduced neurites, while neurites of motor neuron expressing HSPB8-K141N (C) or HSPB8-K141E (D) accumulate more APP (arrow). The incidence of APP accumulation was quantified by counting the proportion of cells with strong neuritic APP accumulation (E). **P-value < 0.01. Scale bar = 10 µm.