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. 2010 Jun 10;19(16):3254–3265. doi: 10.1093/hmg/ddq234

Figure 7.

Figure 7.

Expression of mutant HSPB8 does not result in increased activation of apoptosis in different neuronal and non-neuronal cell types. Activation of enzymes implicated in initiation and execution of apoptosis was examined by western blot of cleaved (17, 19 kDa) and total caspase 3 (35 kDa), cleaved (35, 37 kDa) and total caspase 9 (47 kDa) and cleaved (89 kDa) and total PARP (116 kDa) in different cell types. Stimulation with etoposide (4 h in 50 µM) was used as a positive control for apoptosis activation in the human neuronal cell line SH-SY5Y stably expressing GFP, WT-HSPB8-V5 or mutant K141N/K141E-HSPB8-V5 constructs (A), in rat primary motor neurons (B) and in rat primary glial cells (C) transduced with pLenti-GFP, pLenti-WT-HSPB8-GFP or mutant pLenti-K141N/K141E-HSPB8-GFP constructs. Expression of mutant HSPB8 did not result in increased basal levels or in increased activation after etoposide treatment of the markers for apoptosis. Normalization was performed with β-actin.