Fig. 6.
High-CLIP-affinity Ii reduces unsupervised peptide loading. Presentation of the I-Ag7-restricted 1040-79 (p79) mimetope peptide by stable 3A5.g7 or A20.g7 Ii transfectants was detected by a BDC2.5 T-cell hybridoma. IL-2 production was measured by ELISA. (A) Presentation of exogenously added peptide by 3A5.g7 (DM-) Ii transfectants. One representative experiment of five is shown. Data shown as mean of highly reproducible duplicates. (B and C) Summary of data from multiple experiments. For each antigen concentration, IL-2 production is normalized to wt within the same experiment. (B) Presentation of exogenously added p79 peptide by 3A5.g7 transfectants; n = 5 for 0.1, 1 and 10 μM peptide and n = 2 for 0.01 μM peptide. (C) Presentation of exogenously added p79 peptide by A20.g7 transfectants; n = 5 for 0.1, 1 and 10 μM peptide and n = 4 for 0.3, 3 and 30 μM peptide.