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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Eur J Neurosci. 2010 Jul;32(1):108–117. doi: 10.1111/j.1460-9568.2010.07256.x

Figure 1. LTPGABA is impaired by morphine 2 hours following injection, but recovers after 5 days.

Figure 1

A, Diagram of injection protocol. B, Averaged experiments show that SNAP potentiates IPSCs in slices prepared from saline-treated animals 2 hours after injection (n = 7), but is impaired in morphine-treated animals (n = 9). We analyzed the effect of morphine on LTPGABA using two way ANOVA with the drug treatment (saline vs. morphine) as the between subjects factor and time (in minutes) as the within subjects factor. This analysis revealed significant effects of drug treatment (F1,39 = 99.2, p < 0.001), time (F1,39 = 7.00, p < 0.001) and an interaction between these two factors (F1,39 = 0.11, p < 0.001). C, Averaged experiments show that SNAP potentiation of IPSCs is not significantly different in slices prepared from saline (n = 6) or morphine-treated animals (n = 8) 5 days after a single injection of morphine. Statistical analysis revealed a significant effect of time (F1,39 = 5.29, p < 0.001), but no significant effects of drug treatment (F1,39 = 0.63, p > 0.05) or interaction between the two factors (F1,39 = 0.37, p > 0.05).