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. Author manuscript; available in PMC: 2010 Jul 22.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Mar 5;50(7):3056–3064. doi: 10.1167/iovs.08-2222

Figure 1.

Figure 1

Documentation of CNV development. CNV was induced using laser photocoagulation of Bruch membrane. (A–C) Isolectin-B4 staining, which highlights newly formed murine vessels, is used to document the size increase of the CNV lesion over time. Confocal microscopy, capturing Z-stacks of images through the entire depth of each CNV lesion, was performed using identical laser intensity settings for all experiments. (D, E) Intensity profiles were plotted and analyzed for lesion size (peak fluorescence and area under the curve; integrated fluorescence). (F) Effects of the lesion (or the treatments) on retina function were determined by electroretinography, revealing a decrease in ERG amplitudes for both a- and b-waves (photoreceptor and bipolar cell response, respectively) 6 days after the induction of CNV. (G) The increase in the size of the lesion as determined by confocal microscopy correlated with a decrease in the ERG amplitude (lesions were normalized to maximum size, as determined after 2 weeks; ERG b-waves were expressed as a percentage of baseline amplitudes). Scale bar, 50 μm.