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. Author manuscript; available in PMC: 2010 Jul 22.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Mar 5;50(7):3056–3064. doi: 10.1167/iovs.08-2222

Figure 5.

Figure 5

C3d deposition in the CNV lesion. (A) Comparison of C3d deposition by immunohistochemistry using a C3d antibody directly conjugated to FITC (left), and C3d staining overlaid with the differential interference contrast image of mouse retinas (right) frozen sections. Photographs of sections were taken through a CNV lesion, which can be identified by the damage in the pigmented RPE-choroid. Significant C3d deposition was seen by 12 hours after laser; it remained elevated through day 3 and appeared to decline by day 6. (B) Flatmount analysis of the RPE-choroid showed the anti-C3d labeling to be located preferentially at the edge of the lesion. IS/OS (photoreceptor), inner segment/outer segment; ONL, outer nuclear layer, OPL, outer plexiform layer.