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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Rapid Commun Mass Spectrom. 2010 Feb;24(3):267–275. doi: 10.1002/rcm.4383

MALDI spectra of atriopeptin digest heated at 60°C for 1 hour in the presence of reducing reagent (A) 5 mM DTT; (B) 5 mM TCEP; and (C) MS/MS spectrum of the product of desulfurization at m/z=1277.6 (1+), corresponding to the conversion of cysteine to alanine.

MALDI spectra of atriopeptin digest heated at 60°C for 1 hour in the presence of reducing reagent (A) 5 mM DTT; (B) 5 mM TCEP; and (C) MS/MS spectrum of the product of desulfurization at m/z=1277.6 (1+), corresponding to the conversion of cysteine to alanine.