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. Author manuscript; available in PMC: 2010 Jul 22.
Published in final edited form as: J Immunol. 2009 Nov 30;184(1):369–378. doi: 10.4049/jimmunol.0902110

FIGURE 8.

FIGURE 8

Camp−/− NK cells express normal maturation markers but altered levels of perforin, granzyme B, and SPI-6. A, NK cell maturation is not defective in Camp−/− mice. Splenocytes from wt and Camp−/− mice were stained for NK1.1 and CD3, Mac-1, CD49b, CD43, αV, CD27, and NKG2D. Gated NK1.1+CD3− cell populations are shown. Percentages are given for dot blot quadrants. Representative dot blot quadrants are shown from five wt and five Camp−/− mice analyzed. Gene expression analyzes in isolated NK cells (NK1.1+CD3−) was determined by quantitative PCR or Western blot. NK cell from wt and Camp−/− mice were analyzed for their expression of perforin (B), granzyme B (C), and the physiological inhibitor of granzyme B, SPI-6 (D). Cells were pooled from three to eight mice. Histograms are representative of two to five independent experiments.