Skip to main content
. Author manuscript; available in PMC: 2011 Jul 9.
Published in final edited form as: Mol Cell. 2010 Jul 9;39(1):48–58. doi: 10.1016/j.molcel.2010.06.013

Figure 4.

Figure 4

EGFP-MuB dissociation from the target complex. (A) EGFP signal intensities of 5 kbp (1.6 μm) segments surrounding the locations of MuA clusters were measured before and after buffer flow interruption and the intensity ratio (After/Before) was calculated for each MuA cluster. Distributions of the ratio after buffer flow interruption of 15 sec (yellow), 2 min (green) and 5 min (red) are shown with a Gaussian fit. (B) The intensity ratio distribution was also measured for locations at 5 (blue), 10 (purple) or 15 (orange) kbp away from MuA clusters (center distance of 5 kbp windows) after 5 min buffer flow interruption and compared to the 5 min data in (A) (red). Gaussian fitted curves were normalized relative to the peak value. (C) Representative fluorescence image pairs of individual EGFP-MuB target complexes before (left) and after (center) buffer flow interruption and Alexa-633 marked MuA clusters (right). Arrows indicate the positions of the MuA clusters involved. Brackets indicate the region where EGFP-MuBs were dissociated during buffer flow interruption.