Figure 4. TMV Convert CD25^neg T cells to Treg.

(A) Flow cytometry histograms of cultured (d5) CD4⁺CD25^neg T cells showing conversion of CD25^neg T cells into CD25⁺ T cells ± TMV or DC-derived MV (left panel) and expression of FOXP3 in the converted CD4⁺CD25⁺ T cells (right panel) in the same cultures. (B) A phenotypic profile of CD4⁺CD25^high T cells present in 7 day cultures of CD4⁺CD25⁺ T cells ± TMV or DC-derived MV. T cells were stained with various mAbs and evaluated by multiparameter flow cytometry. The gate is set on CD4⁺CD25^high T cells. The data are mean percentages ± SD of positive cells from three independent experiments. (C) MFI for FasL, IL-10, TGF-β1, granzyme B and perforin expression in CD4⁺CD25^high T cells cultured as described in (B) ± TMV. The data are representative of three independent experiments.