Figure 6. Macrophages, IL-1β and TNFα stabilize Snail in a NF-κB/AKT/Wnt dependent manner.

A: Macrophages, IL-1β and TNFα stabilize Snail in a NF-κB/AKT/Wnt dependent manner. HCT116 cells were transfected with dnIκB, dnAKT or dnTCF4 and were treated with IL-1β or TNFα, or were cultured with THP1 macrophages as indicated. The levels of Snail and c-jun were determined by immunoblotting. B: The levels of Snail and pGSK3β were determined by immunoblotting in Hke-3 cells that were treated with IL-1β, TNFα or were co-cultured with macrophages in the absence or the presence of LY 294002 for 24 hours. C: HCT116 and Hke-3 cells were treated with TRAIL in the absence or the presence of IL-1β and LY294002, as indicated. The experiment was performed three times.