Figure 7. Snail regulates Wnt signaling.

A: HCT116 and Hke-3 cells were transfected with TOP-FLASH reporter together with an empty vector (neo), or the increasing concentrations of Snail expression vector. The experiment was performed in triplicate. B: Tumor cells were transfected with NSP or Snail specific siRNA and were treated with IL-1β as indicated. The levels of Snail were determined by immunoblotting. C: Tumor cells were transfected with TOP-FLASH reporter together with NSP or Snail specific siRNA. Data represent the average of two independent experiments each performed in duplicate.