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. 2010 Jul 7;9:54. doi: 10.1186/1475-2859-9-54

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Copyright ©2010 Kim et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Scheme for construction of the hydrogenase 1 expression vector. Full-length hya operon was cloned into pGEM-T to generate (A) pGemT-EcH3, and two subunit genes (hyaA &hyaB) comprising the core enzyme cloned into pET-21b and pTrcHisC expression vectors to generate (B) pET-EcHAB for the analysis of hydrogen production in recombinant BL21 and (C) pTrc-EcH1ABHis for the analysis of protein expression with His₆-tag, respectively. His₆-tag was fused to each subunit gene in pTrc-EcH1ABHis for facile purification of hydrogenase (Refer to Materials and Methods for scheme of His₆-tag fusion).