Table 2.
Betanodavirus propagation in the medaka cell lines
| Cell line | Inoculum a (TCID50) | Viral titer (TCID50/ml) b | |||
|---|---|---|---|---|---|
| Day 1 | Day 3 | Day 5 | Day 7 | ||
| OLHNI-1 | 103 | 4.4 × 104 ± 1.2 × 104 | 4.9 × 108 ± 7.0 × 107 | 1.8 × 109 ± 7.5 × 108 | NT |
| OLHNI-2 | 103 | 4.0 × 104 ± 0 | 4.0 × 108 ± 1.7 × 108 | 1.4 × 109 ± 4.0 × 108 | NT |
| OLHE-131 | 103 | 3.3 × 103 ± 7.5 × 102 | 4.4 × 107 ± 1.2 × 107 | 2.6 × 108 ± 6.0 × 107 | NT |
| OLKaga-e1 | 103 | 4.0 × 103 ± 0 | 2.9 × 108 ± 3.5 × 107 | 3.2 × 108 ± 8.5 × 107 | NT |
| OLHdrR-e3 | 103 | 4.8 × 103 ± 8.0 × 102 | 1.9 × 108 ± 1.0 × 107 | 2.9 × 108 ± 3.5 × 107 | NT |
| OLCAB-e3 | 106 | 2.9 × 105 ± 3.5 × 104 | 2.1 × 107 ± 4.0 × 106 | 2.5 × 107 ± 1.5 × 107 | 3.6 × 107 ± 4.0 × 106 |
| OLCAB-e21 | 106 | 5.1 × 104 ± 1.9 × 104 | 4.1 × 106 ± 1.6 × 106 | 3.7 × 106 ± 1.9 × 106 | 1.9 × 106 ± 1.3 × 106 |
| OLCAB-e31 | 106 | 3.6 × 105 ± 4.0 × 104 | 5.6 × 106 ± 0 | 5.6 × 106 ± 0 | 7.8 × 106 ± 2.2 × 106 |
| OLF-136 | 106 | 1.1 × 105 ± 6.9 × 104 | 4.8 × 106 ± 8.0 × 105 | 7.8 × 106 ± 2.2 × 106 | 2.1 × 107 ± 1.1 × 107 |
| OLME-104 | 106 | 4.8 × 104 ± 8.0 × 103 | 4.8 × 104 ± 8.0 × 103 | 3.6 × 104 ± 4.0 × 103 | 3.6 × 104 ± 4.0 × 103 |
| E-11 | 103 | 2.7 × 104 ± 1.3 × 104 | 1.4 × 108 ± 1.3 × 108 | 1.4 × 108 ± 4.0 × 107 | NT |
a. Medaka cells and E-11 cells (1.0-1.5 × 105) were inoculated with RGNNV of the indicated titers.
b. Viral titers in the culture supernatant were determined by the TCID50 assay using E-11 cells at 30°C.
Each data represents the mean and standard deviation from two independent experiments.
NT, Not tested.