Fig. 3.

Time course of the effects of isobutylmethylxanthine (IBMX) and PPP on β-III-tubulin expression. Adipose tissue-derived stem cells were treated with IBMX and PPP, as described in Figure 1, and for additional time points at 3 and 24 hr. The cells were then processed for Western blotting with an anti-β-III-tubulin antibody and an anti-β-actin antibody. The resulting radiographs were analyzed by densitometry, and the data by Prism 4 software. For each time point (A, 1 hr; B, 3 hr; C, 24 hr), a representative radiograph is shown on the left and the statistical analysis data on the right. “Relative expression” is the ratio of β-III-tubulin versus β-actin expression. Each bar represents the average of 3 independent experiments. The asterisks above the second bar (induction) indicate significant difference (p<0.01) between “induction” and “control.” The asterisks above the fourth bar (induction+PPP) indicate significant difference (*p<0.05; **p<0.01) between “induction+PPP” and “induction+DMSO.”