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. Author manuscript; available in PMC: 2010 Jul 22.
Published in final edited form as: Differentiation. 2007 Nov 15;76(5):488–494. doi: 10.1111/j.1432-0436.2007.00240.x

Fig. 4.

Fig. 4

Effects of isobutylmethylxanthine (IBMX) and PPP on expression and phosphorylation of Insulin growth factor (IGF)-IR. (A) Adipose tissue-derived stem cells were treated with IBMX and PPP, as described in Figure 1. The cells were then processed for immunofluorescence staining with anti-IGF-IR antibody, anti-phospho-IGF-IR antibody, and diamidino-2-phenylindole. Green fluorescence indicates IGF-IR expression (A) or phospho-IGF-IR expression (B); blue fluorescence indicates cell nucleus. Original magnification was × 200.