Table 3.
Histological Layers | ||||||
---|---|---|---|---|---|---|
Mucosa and Submucosa | Muscular | |||||
Groups | AB+ | SAF+ | AB+ SAF+ | AB+ | SAF+ | AB+ SAF+ |
I (Control) | 7 ± 2 | 0 ± 0 | 3 ± 0 | 1 ± 0 | 1 ± 1 | 2 ± 1 |
II | 12 ± 4 | 0 ± 0 | 2 ± 1 | 2 ± 1 | 2 ± 1 | 11 ± 9 |
III - NT | 40 ± 14* | 1 ± 1 | 4 ± 1 | 8 ± 3* | 3 ± 2 | 7 ± 3 |
III - MT | 12 ± 2 | 0 ± 0 | 3 ± 1 | 11 ± 3* | 4 ± 1 | 10 ± 4 |
IV - NT | 19 ± 3** | 1 ± 0,6 | 12 ±8 | 9 ± 3* | 1 ± 0 | 18 ± 7 |
IV - MT | 27 ± 10 | 9 ± 8 | 25 ± 8#& | 9 ± 2**π | 3 ± 2 | 22 ± 9 |
Histological preparation was performed as described in Materials and Methods. Values (number of cells per mm2) are expressed as mean ± SEM of 60 fields analysed per animal with a high power objective (40×). Alcian-blue (AB+), safranin (SAF+) and alcin-blue/safranin positive cells (AB+SAF+). * P < 0.05 vs. group I; # < 0.05 vs. I and III-MT; & P < 0.01 vs. II; π P < 0.05 vs. II.