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. 2010 Jul 22;5(7):e11716. doi: 10.1371/journal.pone.0011716

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O'Hayre et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Bar graph depicting the spectral counts of HSP27 phosphopeptides (Ser82) observed in the LC-MS/MS analysis after CXCL12 stimulation. B) Western blot detecting phosphorylation of HSP27 and the upstream p38-MAPK, and total HSP27 over time course of 0 to 60 min CXCL12 stimulation (30 nM) from CLL A patient cells and 2 other representative CLL patients' cells. β-actin was run as a loading control. C) Signaling diagram of HSP27, which can protect from apoptosis, and its upstream regulation by p38-MAPK and MAPKAPK2.