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. 2010 Jul 22;6(7):e1001012. doi: 10.1371/journal.ppat.1001012

Figure 11. Knockdown of MFN1 inhibits the redistribution of IPS-1 induced by NDV infection.

Figure 11

A, HeLa cells were transfected with negative control (N.C.) or hMFN1-targeted siRNA (#1–#3) for 48 h, and the knockdown of endogenous MFN1 was analyzed by Western blotting using anti-MFN1 antibody. B, Cells transfected with siRNA as shown in a were infected with NDV for 12 h, and endogenous IFNB1 mRNA expression was quantified by qRT-PCR. Data represent means ± s.d. (n = 3). C, IPS-1-HeLa cells transfected with N.C. or hMFN1-targeted siRNA were infected with NDV for 12 h, and endogenous IFNB1 mRNA expression was quantified by qRT-PCR. Data represent means ± s.d. (n = 3). D, IPS-1-HeLa cells transfected with N.C. or hMFN1-targeted siRNA#2 for 48 h. Cells were infected with NDV for 12 h and stained with anti-FLAG antibody (FLAG-IPS-1), anti-NP antibody (NDV NP), and MitoTracker (Mitochondria).