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ACR.2 Acrosomal fluorescent patterns (FITC-conjugated secondary antibody). Representative pictures of three specific ACR.2 acrosomal fluorescent patterns. (A) Uncapacitated, acrosome intact sperm - moderate uniform fluorescence in the acrosomal area; (B) Capacitated, acrosome-intact sperm - intensive fluorescence of the acrosome; (C) Acrosome-reacted sperm - low or no fluorescent signal in the sperm head. Nuclei stained with a Blue DAPI dye.
