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. 2010 Jul 13;8:87. doi: 10.1186/1477-7827-8-87

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Copyright ©2010 Ded et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Number of sperm after AR evaluated by ACR.2 antibody. After 240 min of capacitation, control and experimental samples from boars A-D with 1 μM concentration of four estrogens were treated by zona pellucida to induce AR. There was a significantly higher number of sperm, which underwent calcium ZP-induced AR in all experimental samples in comparison to the experimental group. All estrogens significantly increased the number of sperm after the ZP-AR. Differences were analyzed by KW-ANOVA; post hoc comparison was performed by multiple comparisons of mean ranks. Whiskers denote ± SE. *P < 0.05, **P < 0.01, ***P < 0.001.