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. 2010 Jul 22;6(7):e1001032. doi: 10.1371/journal.pgen.1001032

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Diagram of a typical S. pombe mating-type (MT) locus (not to scale). Symbols: cen2 –centromere2, RTS1-polar replication fork barrier, DSB- imprinting site where DSB forms during replication; purple arrows - inverted repeats. Primer names correspond to distance in kilobases from the DSB. (B) ChIP-qPCR analysis of γH2A distribution at the MT locus in G2 and S phase. Cells were synchronized in G2 phase using the cdc25-22 allele and S-phase progression was monitored using septation index. ChIP enrichment at the indicated sites was quantitated %IP.