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. 2010 Jul 22;5(7):e11717. doi: 10.1371/journal.pone.0011717

Figure 1. Response of steroid hormone receptors in MMTV-reporter gene assays.

Figure 1

Neuronal SK-N-MC cells were transfected with a plasmid expressing one of the HA-tagged SRs, the MMTV firefly-luciferase reporter plasmid when transfecting GR, MR, PR, or AR, an ERE firefly-luciferase reporter plasmid for ERα and ERβ, and the Gaussia-KDEL control plasmid. After transfection, the cells were cultivated for 24 h in the presence of the indicated concentrations of hormone (DHT: Dihydrotestosterone) or EtOH as solvent control. Receptor activity represents firefly data normalized to Gaussia activities + S.E.M. of at least four independent experiments, each performed in duplicate.