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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Immunobiology. 2010 Mar 4;215(12):941–948. doi: 10.1016/j.imbio.2010.02.004

Figure 2.

Figure 2

Induction of Th-1 dominant reovirus-specific humoral and cell-mediated immune responses. A. Reovirus-specific serum IgG2a isotype antibody titers from orally treated mice were determined as described in materials and methods. Results shown are combined from two independent experiments. Error bars indicate standard error among 7-10 mice per group. B. Splenic CD8+ T-cells were harvested from reovirus infected mice and restimulated in vitro with reovirus. Activation of CD8+ effector T-cells was assayed by the production of intracellular IFN-γ when challenged with non-infected (open bars) or reovirus-infected (filled bars) L929 mouse fibroblast cell. Bars represent the percentage of the maximum IFN-γ response of CD8+ T-cells following in vitro stimulation. Maximum response of CD8+ T-cells was determined through polyclonal activation with anti-CD3ε/CD28 antibodies. Error bars indicate standard deviation of two replicates. Data were analyzed for statistical significance by ANOVA followed by Tukey’s post hoc test, (*) p<0.05, (**) p<0.01.